Abstract
Stem cells are nowadays isolated from multiple sources and used in biotechnological application. Our study presents a new method for isolation and expansion of stem cells derived from infrapatellar fat pad (IPFP) by simple adherence to plastic surface of culture flasks. This method yielded adipose-derived stromal cells (ASC) with morphological and phenotypical characteristics of mesenchymal stem cells, without using any enzymatic digestion, making these cells more suitable for medical application, in treatment of different human pathologies.
Keywords: stem cells; infrapatellar fat pad (IPFP); adipose stem cells (ASC); plastic adherence