Abstract
The aim of this study was to demonstrate the use of adenosine triphosphate– (ATP–) driven bioluminescence as an innovative tool for the rapid chairside enumeration of oral bacteria (including plaque streptococci) and assessment of oral hygiene and caries risk. The study group included 60 patients with 120 direct coronal restorations with three tipes of restorative materials (resin-modified glass ionomer cement GC Fuji VIII, giomer Beautifill Flow Plus Shofu and composite resin (GC Gradia) from witch we have collected 120 specimens using a luciferase-based assay system(system SURE II). The values of ATP were obtained with System SURE II device and statisticaly analised with Wilcoxon Test. The lowest value was shown for glass ionomer cement GC Fuji VIII, comparing with composite resins, but in time we have seen the increase of ATP for all three restorative materials.
Keywords: ATP; restorative materials; oral biofilm; caries risk