Abstract
The purpose of this work was the detailed study of morphological biodegradation and the citotoxicity effect of some experimental biomaterials. The study has four experimental biomaterials based on polylactic acid, polyethylene glycol, tricalcium phosphate, chitosan and hydroxyapatite in different percent cured in air followed by heat treatment at 1200C. The experimental biomaterials were characterized by AFM (Multimode V-SPM, Veeco) and SEM (Q130 -FEI Company) microscopy. For the citotoxicity were used cell cultures of normal human fibroblasts and the viability testing was made with trypan blue. According to the experimental results, the biomaterial with highest percent in polylactic acid presents a high degradation, compared with the rest of the biomaterials which have HA in composition, who decrease this process. Fibroblasts cell culture showed good adhesion to the surface of the biomaterials. The viability of the biomaterials increases at 48h compared with 24h, which suggest the absence of the materials cytotoxicity. Our results sustain idea that biomaterials based on chitosan with additions can be successfully used for further studies in reparative medicine. Keywords: chitosan, biodegradable polymers, hydroxyapatite, SEM, AFM, citotoxicity