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Materiale Plastice
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Materiale Plastice (Mater. Plast.), Year 2020, Volume 57, Issue 1, 223-235

https://doi.org/10.37358/MP.20.1.5331

Puja K Gangurde, Navya Ajitkumar Bhaskaran, Ruchi Verma, Jobin Jose, Lalit Kumar

Analytical Method Development Using Central Composite Design for Estimation of Lamotrigine in Lipid Nanoformulation


Abstract:
Objective of this study was to develop and validate HPLC-UV method for detection of LTG in lipid nanoformulations. HPLC-UV method was developed according to ICH Q2(R1) guidelines. Central composite design was used effectively to optimize and study the effect of buffer strength, flow rate, pH of buffer and mobile phase composition on responses such as tailing factor, peak area, retention time and number of theoretical plates. The 30 mM ammonium formate buffer and acetonitrile (in the ratio 65:35 %v/v) was used as mobile phase in the study. The mobile phase was delivered at the flow rate of 1.0 mL/min. The detection of buffer was performed at 256 nm using UV detector. The drug entrapment of prepared formulation was also determined using developed HPLC method. Retention time of lamotrigine was found to be 3.844 min. The coefficient of determination (r2) value from linearity was found to be 0.9982. Percent relative standard deviation value of precision was found to be within the acceptable range. The estimated LOD and LOQ were found to be 9.07 ng/mL and 27.48 ng/mL, respectively. Drug entrapment of prepared lipid nanoformulation was found to be 73.44 ± 6.65%. The results conclude that the developed analytical method is simple, precise, sensitive, fast and reproducible. Applications of developed method for determination of drug entrapment in prepared lipid nanoformulation confirmed that the developed analytical method is suitable for estimation of lamotrigine in lipid nanoformulations.


Keywords:
Lamotrigine; RP-HPLC-UV; central composite design; lipid nanoformulations; design of experiments

Issue: 2020 Volume 57, Issue 1
Pages: 223-235
Publication date: 17/04/2020
https://doi.org/10.37358/MP.20.1.5331
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Creative Commons License
This article is published under the Creative Commons Attribution 4.0 International License
Citation Styles
Cite this article as:
GANGURDE, P.K., BHASKARAN, N.A., VERMA, R., JOSE, J., KUMAR, L., Analytical Method Development Using Central Composite Design for Estimation of Lamotrigine in Lipid Nanoformulation, Mater. Plast., 57(1), 2020, 223-235.

Vancouver
Gangurde PK, Bhaskaran NA, Verma R, Jose J, Kumar L. Analytical Method Development Using Central Composite Design for Estimation of Lamotrigine in Lipid Nanoformulation. Mater. Plast.[internet]. 2020 Jan;57(1):223-235. Available from: https://doi.org/10.37358/MP.20.1.5331


APA 6th edition
Gangurde, P.K., Bhaskaran, N.A., Verma, R., Jose, J. & Kumar, L. (2020). Analytical Method Development Using Central Composite Design for Estimation of Lamotrigine in Lipid Nanoformulation. Materiale Plastice, 57(1), 223-235. https://doi.org/10.37358/MP.20.1.5331


Harvard
Gangurde, P.K., Bhaskaran, N.A., Verma, R., Jose, J., Kumar, L. (2020). 'Analytical Method Development Using Central Composite Design for Estimation of Lamotrigine in Lipid Nanoformulation', Materiale Plastice, 57(1), pp. 223-235. https://doi.org/10.37358/MP.20.1.5331


IEEE
P.K. Gangurde, N.A. Bhaskaran, R. Verma, J. Jose, L. Kumar, "Analytical Method Development Using Central Composite Design for Estimation of Lamotrigine in Lipid Nanoformulation". Materiale Plastice, vol. 57, no. 1, pp. 223-235, 2020. [online]. https://doi.org/10.37358/MP.20.1.5331


Text
Puja K Gangurde, Navya Ajitkumar Bhaskaran, Ruchi Verma, Jobin Jose, Lalit Kumar,
Analytical Method Development Using Central Composite Design for Estimation of Lamotrigine in Lipid Nanoformulation,
Materiale Plastice,
Volume 57, Issue 1,
2020,
Pages 223-235,
ISSN 2668-8220,
https://doi.org/10.37358/MP.20.1.5331.
(https://revmaterialeplastice.ro/Articles.asp?ID=5331)
Keywords: Lamotrigine; RP-HPLC-UV; central composite design; lipid nanoformulations; design of experiments


RIS
TY - JOUR
T1 - Analytical Method Development Using Central Composite Design for Estimation of Lamotrigine in Lipid Nanoformulation
A1 - Gangurde, Puja K
A2 - Bhaskaran, Navya Ajitkumar
A3 - Verma, Ruchi
A4 - Jose, Jobin
A5 - Kumar, Lalit
JF - Materiale Plastice
JO - Mater. Plast.
PB - Materiale Plastice SRL
SN - 2668-8220
Y1 - 2020
VL - 57
IS - 1
SP - 223
EP - 235
UR - https://doi.org/10.37358/MP.20.1.5331
KW - Lamotrigine
KW - RP-HPLC-UV
KW - central composite design
KW - lipid nanoformulations
KW - design of experiments
ER -


BibTex
@article{MatPlast2020P223,
author = {Gangurde Puja K and Bhaskaran Navya Ajitkumar and Verma Ruchi and Jose Jobin and Kumar Lalit},
title = {Analytical Method Development Using Central Composite Design for Estimation of Lamotrigine in Lipid Nanoformulation},
journal = {Materiale Plastice},
volume = {57},
number = {1},
pages = {223-235},
year = {2020},
issn = {2668-8220},
doi = {https://doi.org/10.37358/MP.20.1.5331},
url = {https://revmaterialeplastice.ro/Articles.asp?ID=5331}
}

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