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Materiale Plastice
Cite as: Mater. Plast.
https://doi.org/10.37358/Mat.Plast.1964

OSIM Nr. R102356
ISSN Print 0025-5289
ISSN Online 2668-8220
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Materiale Plastice (Mater. Plast.), Year 2018, Volume 55, Issue 3, 303-307

https://doi.org/10.37358/MP.18.3.5018

Andrei Marian Feier, Doina Ramona Manu, Gabriela Strnad, Minodora Dobreanu, Octav Marius Russu, Diana Portan, Tiberiu Bataga

A Step Forward Standardization of Biocompatibility Testing on Tissue Culture Polystyrene


Abstract:
Standard procedures regarding osteoblast cells deposition on different subtrates to test the biocompatibility of materials are not available. Aspects related to cells morphology depending on their number on the surface area of the substrate were studied for the first time. Osteoblasts were successfully isolated from residual bone resulted from two different patients after total hip arthroplasty. Different numbers of osteoblasts in populations (3x104 cells/cm2, 6x104 cells/cm2, 1x105 cells/cm2) were deposited on tissue culture polystyrene for 7 and 10 days of incubation. Results suggested that the protocol of osteoblasts deposition should be adjusted to assure a fair distribution of cells on the entire available area of the substrate, possible if a very good dispersion in the medium is achieved prior to deposition. Some other key factors in cells appropriate development, such as substrate nature, topography and incubation time,were detected. The present investigation helps declaring specific standards with respect to the biocompatibility testing of materials using osteoblast populations. Further investigations may lead to the creation of databases and the establishment of standards regarding bicompatibility researches that involve osteoblasts populations.


Keywords:
tissue culture polystyrene; osteoblasts populations; biocompatibility standards

Issue: 2018 Volume 55, Issue 3
Pages: 303-307
Publication date: 2018/9/30
https://doi.org/10.37358/MP.18.3.5018
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Creative Commons License
This article is published under the Creative Commons Attribution 4.0 International License
Citation Styles
Cite this article as:
FEIER, A.M., MANU, D.R., STRNAD, G., DOBREANU, M., RUSSU, O.M., PORTAN, D., BATAGA, T., A Step Forward Standardization of Biocompatibility Testing on Tissue Culture Polystyrene, Mater. Plast., 55(3), 2018, 303-307.

Vancouver
Feier AM, Manu DR, Strnad G, Dobreanu M, Russu OM, Portan D, Bataga T. A Step Forward Standardization of Biocompatibility Testing on Tissue Culture Polystyrene. Mater. Plast.[internet]. 2018 Jul;55(3):303-307. Available from: https://doi.org/10.37358/MP.18.3.5018


APA 6th edition
Feier, A.M., Manu, D.R., Strnad, G., Dobreanu, M., Russu, O.M., Portan, D. & Bataga, T. (2018). A Step Forward Standardization of Biocompatibility Testing on Tissue Culture Polystyrene. Materiale Plastice, 55(3), 303-307. https://doi.org/10.37358/MP.18.3.5018


Harvard
Feier, A.M., Manu, D.R., Strnad, G., Dobreanu, M., Russu, O.M., Portan, D., Bataga, T. (2018). 'A Step Forward Standardization of Biocompatibility Testing on Tissue Culture Polystyrene', Materiale Plastice, 55(3), pp. 303-307. https://doi.org/10.37358/MP.18.3.5018


IEEE
A.M. Feier, D.R. Manu, G. Strnad, M. Dobreanu, O.M. Russu, D. Portan, T. Bataga, "A Step Forward Standardization of Biocompatibility Testing on Tissue Culture Polystyrene". Materiale Plastice, vol. 55, no. 3, pp. 303-307, 2018. [online]. https://doi.org/10.37358/MP.18.3.5018


Text
Andrei Marian Feier, Doina Ramona Manu, Gabriela Strnad, Minodora Dobreanu, Octav Marius Russu, Diana Portan, Tiberiu Bataga,
A Step Forward Standardization of Biocompatibility Testing on Tissue Culture Polystyrene,
Materiale Plastice,
Volume 55, Issue 3,
2018,
Pages 303-307,
ISSN 2668-8220,
https://doi.org/10.37358/MP.18.3.5018.
(https://revmaterialeplastice.ro/Articlegs.asp?ID=5018)
Keywords: tissue culture polystyrene; osteoblasts populations; biocompatibility standards


RIS
TY - JOUR
T1 - A Step Forward Standardization of Biocompatibility Testing on Tissue Culture Polystyrene
A1 - Feier, Andrei Marian
A2 - Manu, Doina Ramona
A3 - Strnad, Gabriela
A4 - Dobreanu, Minodora
A5 - Russu, Octav Marius
A6 - Portan, Diana
A7 - Bataga, Tiberiu
JF - Materiale Plastice
JO - Mater. Plast.
PB - Materiale Plastice SRL
SN - 2668-8220
Y1 - 2018
VL - 55
IS - 3
SP - 303
EP - 307
UR - https://doi.org/10.37358/MP.18.3.5018
KW - tissue culture polystyrene
KW - osteoblasts populations
KW - biocompatibility standards
ER -


BibTex
@article{MatPlast2018P303,
author = {Feier Andrei Marian and Manu Doina Ramona and Strnad Gabriela and Dobreanu Minodora and Russu Octav Marius and Portan Diana and Bataga Tiberiu},
title = {A Step Forward Standardization of Biocompatibility Testing on Tissue Culture Polystyrene},
journal = {Materiale Plastice},
volume = {55},
number = {3},
pages = {303-307},
year = {2018},
issn = {2668-8220},
doi = {https://doi.org/10.37358/MP.18.3.5018},
url = {https://revmaterialeplastice.ro/Articlegs.asp?ID=5018}
}
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