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Materiale Plastice
Cite as: Mater. Plast.
https://doi.org/10.37358/Mat.Plast.1964

OSIM Nr. R102356
ISSN Print 0025-5289
ISSN Online 2668-8220
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Materiale Plastice (Mater. Plast.), Year 2017, Volume 54, Issue 3, 523-526

https://doi.org/10.37358/MP.17.3.4886

Gabriela Tanasie, Florina Bojin, Romulus Fabian Tatu, Alina Maria Sisu, Mirabela Cristea, Daniela Anca Puscasiu, Emanuel Adelin Nemes, Carmen Sofica Tatu

In Vitro Effects of Biomaterials on Mesenchymal Stem Cells Viability and Proliferation


Abstract:
Mesenchymal stem cells (MSCs) are increasingly being used in regenerative medicine to repair various tissue types. This study aimed to assess changes in MSCs properties as a result of their interaction with two biomaterials in order to evaluate their in vitro compatibility. We have used two types of biomaterials, as follows: hydroxyapatite anodized titanium (HA-Ti) is used in bone reconstruction; it is hard and remains in the body, while patches of collagen combined with poly-e-caprolactone (C-PCL) are utilized in the reconstruction of soft tissue (particularly the skin); these patches resorb in the body, being only a temporary matrix that fosters and accelerates tissue formation. We have chosen to assess changes in morphology, viability and proliferation of MSCs obtained by lipoaspirate, through direct contact with the biomaterial or in eluate. MSCs properties were assessed by optical microscopy, fluorescence microscopy after acridine orange/ethidium bromide (AO/EB) staining, and the MTT assay. After result interpretation, we gained an image of the cytotoxicity phenomenon seen during the experiment and were able to assess viability of cell in contact with the biomaterials used. HA-Ti shows better interaction with cells, which preserve their viability, and does not influence MSCs growth, proliferation or morphology. In contrast, cells on C-PCL matrix become small, spherical, were detached from the plate and had lower viability percentage.


Keywords:
regenerative medicine; mesenchymal stem cells; biomaterials; titanium plates; collagen patches

Issue: 2017 Volume 54, Issue 3
Pages: 523-526
Publication date: 2017/9/30
https://doi.org/10.37358/MP.17.3.4886
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Creative Commons License
This article is published under the Creative Commons Attribution 4.0 International License
Citation Styles
Cite this article as:
TANASIE, G., BOJIN, F., TATU, R.F., SISU, A.M., CRISTEA, M., PUSCASIU, D.A., NEMES, E.A., TATU, C.S., In Vitro Effects of Biomaterials on Mesenchymal Stem Cells Viability and Proliferation, Mater. Plast., 54(3), 2017, 523-526.

Vancouver
Tanasie G, Bojin F, Tatu RF, Sisu AM, Cristea M, Puscasiu DA, Nemes EA, Tatu CS. In Vitro Effects of Biomaterials on Mesenchymal Stem Cells Viability and Proliferation. Mater. Plast.[internet]. 2017 Jul;54(3):523-526. Available from: https://doi.org/10.37358/MP.17.3.4886


APA 6th edition
Tanasie, G., Bojin, F., Tatu, R.F., Sisu, A.M., Cristea, M., Puscasiu, D.A., Nemes, E.A. & Tatu, C.S. (2017). In Vitro Effects of Biomaterials on Mesenchymal Stem Cells Viability and Proliferation. Materiale Plastice, 54(3), 523-526. https://doi.org/10.37358/MP.17.3.4886


Harvard
Tanasie, G., Bojin, F., Tatu, R.F., Sisu, A.M., Cristea, M., Puscasiu, D.A., Nemes, E.A., Tatu, C.S. (2017). 'In Vitro Effects of Biomaterials on Mesenchymal Stem Cells Viability and Proliferation', Materiale Plastice, 54(3), pp. 523-526. https://doi.org/10.37358/MP.17.3.4886


IEEE
G. Tanasie, F. Bojin, R.F. Tatu, A.M. Sisu, M. Cristea, D.A. Puscasiu, E.A. Nemes, C.S. Tatu, "In Vitro Effects of Biomaterials on Mesenchymal Stem Cells Viability and Proliferation". Materiale Plastice, vol. 54, no. 3, pp. 523-526, 2017. [online]. https://doi.org/10.37358/MP.17.3.4886


Text
Gabriela Tanasie, Florina Bojin, Romulus Fabian Tatu, Alina Maria Sisu, Mirabela Cristea, Daniela Anca Puscasiu, Emanuel Adelin Nemes, Carmen Sofica Tatu,
In Vitro Effects of Biomaterials on Mesenchymal Stem Cells Viability and Proliferation,
Materiale Plastice,
Volume 54, Issue 3,
2017,
Pages 523-526,
ISSN 2668-8220,
https://doi.org/10.37358/MP.17.3.4886.
(https://revmaterialeplastice.ro/Articlegs.asp?ID=4886)
Keywords: regenerative medicine; mesenchymal stem cells; biomaterials; titanium plates; collagen patches


RIS
TY - JOUR
T1 - In Vitro Effects of Biomaterials on Mesenchymal Stem Cells Viability and Proliferation
A1 - Tanasie, Gabriela
A2 - Bojin, Florina
A3 - Tatu, Romulus Fabian
A4 - Sisu, Alina Maria
A5 - Cristea, Mirabela
A6 - Puscasiu, Daniela Anca
A7 - Nemes, Emanuel Adelin
A8 - Tatu, Carmen Sofica
JF - Materiale Plastice
JO - Mater. Plast.
PB - Materiale Plastice SRL
SN - 2668-8220
Y1 - 2017
VL - 54
IS - 3
SP - 523
EP - 526
UR - https://doi.org/10.37358/MP.17.3.4886
KW - regenerative medicine
KW - mesenchymal stem cells
KW - biomaterials
KW - titanium plates
KW - collagen patches
ER -


BibTex
@article{MatPlast2017P523,
author = {Tanasie Gabriela and Bojin Florina and Tatu Romulus Fabian and Sisu Alina Maria and Cristea Mirabela and Puscasiu Daniela Anca and Nemes Emanuel Adelin and Tatu Carmen Sofica},
title = {In Vitro Effects of Biomaterials on Mesenchymal Stem Cells Viability and Proliferation},
journal = {Materiale Plastice},
volume = {54},
number = {3},
pages = {523-526},
year = {2017},
issn = {2668-8220},
doi = {https://doi.org/10.37358/MP.17.3.4886},
url = {https://revmaterialeplastice.ro/Articlegs.asp?ID=4886}
}
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