REHAB YASSIN GAREEB, MOHAMED SALAH ELNOUBY, MOAUYED AZIZ HASAN, SIMONA TICU (COTORCEA), AMORIN POPA, SIMONA BUNGAU, ELSAYED ELSAYED HAFEZ NEW TREND FOR USING THE REDUCED GRAPHENE OXIDE AS EFFECTIVE AND ECO-FRIENDLY NEMATICIDE Reduced graphene oxide (r-GO) was synthesized chemically and it was characterized using Scanning Electron Microscope (SEM), Transmission Electron Microscope (TEM) and X-Ray Diffraction (XRD). The results revealed that the product r-GO is nano-sheets with non-smooth surface; the size of the obtained nano-sheets ranged from 20 to 100 nm. The well characterized nano-sheets were subjected to cytotoxicity test; results demonstrated that the nano-sheets show low cytotoxicity on the human cell line compared with the chemical nematicide. The r-GO was tested against the nematode during their life span and it was observed that the r-GO is capable to reduce the nematode gall number and egg mass of 94% and 99%, respectively. Moreover, the toxicity of the r-GO against the Juvenile (J2) root-knot nematode (Meloidogyne incognita) was also examined either in laboratory or under the greenhouse conditions. On the other hand, the treatment with the r-GO under greenhouse conditions showed higher mortality of the J2 of 98.5% compared with nematicidal treatment of 96% in a soil infested with M. incognita. Also, the same treatment was sufficient in inducting the growth of tomato plants: fresh weight of the shoot system increased with 30% and of the root system increased up to 285%; dry weight of the shoot system increased with 128% and of the root system increased up to 480%. r-GO has a high nematicidal activity and it is safe for human. The r-Go could be used as safe nematocide because it is safe, cheep, could be produced at large scale, and it is a good additive for the soil.